A simplified method for producing laboratory grade recombinant TEV protease from E. coli.

The tobacco etch virus (TEV) protease has become a popular choice for cleaving fusion proteins because of its high stringency in sequence recognition. Procedures for isolating recombinant protein from the cytoplasm of E. coli require rupturing of the cell wall via enzymatic treatment combined with sonication or French press.

Here we present an expedited method for producing laboratory-grade TEV protease in E. coli using a freeze-thaw method, followed by purification with immobilized metal affinity chromatography.

Porcine IS ELISA Kit

EPI0046 96Tests
EUR 521

Rat IS ELISA Kit

ERI0046 96Tests
EUR 521

Rabbit IS ELISA Kit

ERTI0046 96Tests
EUR 521

Anserini IS ELISA Kit

EAI0046 96Tests
EUR 521

Goat IS ELISA Kit

EGTI0046 96Tests
EUR 521

Canine IS ELISA Kit

ECI0046 96Tests
EUR 521

Chicken IS ELISA Kit

ECKI0046 96Tests
EUR 521

Human IS ELISA Kit

EHI0046 96Tests
EUR 521

Bovine IS ELISA Kit

EBI0046 96Tests
EUR 521

IS ELISA KIT|Human

EF007501 96 Tests
EUR 689

Guinea Pig IS ELISA Kit

EGI0046 96Tests
EUR 521

Rabbit Anti-Mouse Granulocyte Colony Stimulating Factor (GCSF, G-CSF) protein IgG, aff pure

GCSF11-A 100 ug
EUR 482

Rabbit Anti-Human Granulocyte Colony Stimulating Factor (GCSF, G-CSF) protein IgG, aff pure

GCSF12-A 100 ug
EUR 482

Goat Anti-Human Apolipoprotein A-IV A(ApoA4) peptid IgG, aff pure

APOA45-A 100 ul
EUR 482

Human Serum Indoxyl Sulfate (IS) ELISA Kit

abx257961-96tests 96 tests
EUR 746

Human IS(serum indoxyl sulfate) ELISA Kit

EH4913 96T
EUR 567.6
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75 ng/ml

Rabbit Anti-human MART-1/Melanocyte Differentiation-A/Melan-A peptide (CT) IgG

MART12-A 100 ul
EUR 445

Boc-a-Me-DL-Val-OH

A-4145.0001 1.0g
EUR 139
Description: Sum Formula: C11H21NO4; CAS# [139938-00-4]

Boc-a-Me-DL-Val-OH

A-4145.0005 5.0g
EUR 477
Description: Sum Formula: C11H21NO4; CAS# [139938-00-4]

Rabbit Anti-Influenza A HA (H1N1, 1-343aa) (A/Wyoming/3/03) protein IgG, aff pure

H3N22-A 100 ul
EUR 445

Fmoc-a-amino-DL-Gly(Boc)-OH

A-4320.0001 1.0g
EUR 273
Description: Sum Formula: C22H24N2O6; CAS# [176039-39-7]

Fmoc-a-amino-DL-Gly(Boc)-OH

A-4320.0005 5.0g
EUR 998
Description: Sum Formula: C22H24N2O6; CAS# [176039-39-7]

Chicken Anti-Protein A IgG, aff pure

PRTA11-A 1 mg
EUR 482

Goat Anti-Protein-A IgG aff pure

PRTA12-A 0.1 ml
EUR 408

Chicken Anti-Protein-A IgG aff pure

PRTA13-A 100 ug
EUR 408

Rabbit Anti-Influenza A nucleoprotein (H1N1-NP/1-320aa) protein (A/06/California/2009) IgG, aff pure

H1NP21-A 100 ul
EUR 445

Rabbit Anti-Influenza A HA2 (H5N1) (A/Vietnam/1203/2004 366-531aa, >95%, his-tag) protein IgG

HA2H012-A 100 ul
EUR 445

Rabbit Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG, aff pure

H3N23-A 100 ul
EUR 445

Goat Anti-human Serum Amyloid A (SAA) antiserum

SAA11-A 100 ul
EUR 482

Rabbit Anti-Streptococcus A IgG, aff pure, #2

STA12-A 1 mg
EUR 286

Goat Anti-Hepatitis A Virus (Strain HM175) IgG

HAV11-A 100 ul
EUR 457

Monocyte/Granulocyte Antibody

abx415381-01mg 0.1 mg
EUR 453

Rabbit Anti-Human Calcineurin A-subunit IgG aff pure

CALNA12-A 100 ug
EUR 482

Chicken Anti-Human+Mouse IgG+A+M IgG/Y

HGAM11-A 100 ul
EUR 482

Hematoxylin, Weigert's Iron (Part A)

HWI-A-250 250 ml
EUR 116

Rabbit Anti-Mouse a-1-Acid Glycoprotein IgG, aff pure

A1AG18-A 100 ug
EUR 482

Rabbit Anti-Rat a-1-Acid Glycoprotein IgG, aff pure

A1AG19-A 100 ug
EUR 482

Rabbit Anti-Mouse prepro Orexin A IgG # 1, aff pure

PPOX11-A 100 ul
EUR 482

Rabbit Anti-Rat/Human Orexin-A IgG # 1, aff pure

OXA11-A 100 ug
EUR 482

Goat Anti-Green Fluorescent Proteins (GFP, A. victoria) protein, IgG

GFP11-A 100 ul
EUR 408

Rabbit Anti-Human Apolipoprotein A-I protein IgG, aff pure

APOA12-A 100 ug
EUR 482

Rabbit Anti-Mouse Apolipoprotein A-I protein IgG, aff pure

APOA13-A 100 ug
EUR 482

Goat Anti-Human Apolipoprotein A-Il protein IgG, aff pure

APOA21-A 100 ug
EUR 482

Rabbit Phosphorylase b kinase regulatory subunit alpha, liver is

ELI-08786Ra 96 Tests
EUR 928

Rabbit Anti-Influenza A hemeagglutinin A2 (HA2/stem or stalk domain/347-523aa) protein (A/Viet Nam/1203/2004(H5N1) IgG, aff pure

HA28-A 100 ul
EUR 445

Rabbit Anti-Hemagglutinin HA1 Influenza A Virus (H11N2; A/duck/Yangzhou/906/2002) IgG

H11N2-01-A 100 ul
EUR 482

Rabbit Anti-bovine Calcineurin (A+B subunits) protein, IgG, aff pure

CALN11-A 100 ug
EUR 482

Rabbit Anti-Borrelia burgdorferi Osp-A protein (OspA) IgG, aff pure

OSPA12-A 100 ul
EUR 482

Rabbit Anti-Hemagglutinin Influenza A Virus H1N1 H1 (H1N1) (A/New Caledonia/20/99) IgG

H1N1-01-A 100 ul
EUR 482

Rabbit Anti-Rat neurite outgrowth inhibitor (Nogo-A) IgG #1, aff. pure

NogoA11-A 100 ug
EUR 482

Goat Anti-Human/mouse/rat Insulin chain A peptide IgG, aff pure

INSA21-A 100 ul
EUR 482

Monocyte/Granulocyte Antibody (FITC)

abx415380-01mg 0.1 mg
EUR 648

Monocyte/Granulocyte Antibody (RPE)

abx415382-01mg 0.1 mg
EUR 718

Rabbit Anti-Rat Epithelial Na Channel alpha (ENAC-a) IgG #1, Aff. Pure

ENACa11-A 100 ug
EUR 482

Goat Anti-Human recombinant Fetuin A (Alpha-2 HS-Glycoprotein, AHSG, A2HS) IgG

FETB12-A 100 ug
EUR 482

Rabbit Anti-Human Neurite outgrowth inhibitor (Nogo A-C) IgG #2, aff. pure

NogoA12-A 100 ug
EUR 482

Rabbit Anti-Influenza A virus HA IgG (aff pure)

AB-23091-A 100ug
EUR 482

Granulocyte Colony Stimulating Factor Antibody

20-abx137008
  • EUR 356.00
  • EUR 523.00
  • 0.5 mg
  • 1 mg

Granulocyte Marker(BM-1) Antibody

BNCA0061-250 250uL
EUR 383
Description: Primary antibody against Granulocyte Marker(BM-1), APC conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNCA0062-250 250uL
EUR 383
Description: Primary antibody against Granulocyte Marker(BM-2), APC conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNCAP0061-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNCAP0061-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNCAP0062-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNCAP0062-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC810061-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF680R conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC810061-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF680R conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC810062-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF680R conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC810062-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF680R conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC880061-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF488A conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC880061-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF488A conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC880062-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF488A conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC880062-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF488A conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNCB0061-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), Biotin conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNCB0061-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), Biotin conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNCB0062-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), Biotin conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNCB0062-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), Biotin conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC550061-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF555 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC550061-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF555 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC550062-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF555 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC550062-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF555 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC470061-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF647 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC470061-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF647 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC470062-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF647 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC470062-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF647 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC940061-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF594 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC940061-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF594 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC940062-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF594 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC940062-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF594 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNCP0061-250 250uL
EUR 383
Description: Primary antibody against Granulocyte Marker(BM-1), PerCP conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNCP0062-250 250uL
EUR 383
Description: Primary antibody against Granulocyte Marker(BM-2), PerCP conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNCR0061-250 250uL
EUR 383
Description: Primary antibody against Granulocyte Marker(BM-1), RPE conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNCR0062-250 250uL
EUR 383
Description: Primary antibody against Granulocyte Marker(BM-2), RPE conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC040061-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF405S conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody

BNC040061-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF405S conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC040062-100 100uL
EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF405S conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody

BNC040062-500 500uL
EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF405S conjugate, Concentration: 0.1mg/mL

Protease is obtained by expression from the pDZ2087 plasmid in BL21 (DE3) cells. Proteolysis resulting from this product, cleaves a maltose-binding protein fusion to completion at a fusion-to-protease molar ratio of 50:1.

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