The tobacco etch virus (TEV) protease has become a popular choice for cleaving fusion proteins because of its high stringency in sequence recognition. Procedures for isolating recombinant protein from the cytoplasm of E. coli require rupturing of the cell wall via enzymatic treatment combined with sonication or French press.
Here we present an expedited method for producing laboratory-grade TEV protease in E. coli using a freeze-thaw method, followed by purification with immobilized metal affinity chromatography.
Porcine IS ELISA Kit |
EPI0046 |
Abclonal |
96Tests |
EUR 521 |
Rat IS ELISA Kit |
ERI0046 |
Abclonal |
96Tests |
EUR 521 |
Rabbit IS ELISA Kit |
ERTI0046 |
Abclonal |
96Tests |
EUR 521 |
Anserini IS ELISA Kit |
EAI0046 |
Abclonal |
96Tests |
EUR 521 |
Goat IS ELISA Kit |
EGTI0046 |
Abclonal |
96Tests |
EUR 521 |
Canine IS ELISA Kit |
ECI0046 |
Abclonal |
96Tests |
EUR 521 |
Chicken IS ELISA Kit |
ECKI0046 |
Abclonal |
96Tests |
EUR 521 |
Human IS ELISA Kit |
EHI0046 |
Abclonal |
96Tests |
EUR 521 |
Bovine IS ELISA Kit |
EBI0046 |
Abclonal |
96Tests |
EUR 521 |
Guinea Pig IS ELISA Kit |
EGI0046 |
Abclonal |
96Tests |
EUR 521 |
Rabbit Anti-Mouse Granulocyte Colony Stimulating Factor (GCSF, G-CSF) protein IgG, aff pure |
GCSF11-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Rabbit Anti-Human Granulocyte Colony Stimulating Factor (GCSF, G-CSF) protein IgG, aff pure |
GCSF12-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Goat Anti-Human Apolipoprotein A-IV A(ApoA4) peptid IgG, aff pure |
APOA45-A |
Alpha Diagnostics |
100 ul |
EUR 482 |
Human Serum Indoxyl Sulfate (IS) ELISA Kit |
abx257961-96tests |
Abbexa |
96 tests |
EUR 746 |
|
Human IS(serum indoxyl sulfate) ELISA Kit |
EH4913 |
FN Test |
96T |
EUR 567.6 |
|
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75 ng/ml |
Rabbit Anti-human MART-1/Melanocyte Differentiation-A/Melan-A peptide (CT) IgG |
MART12-A |
Alpha Diagnostics |
100 ul |
EUR 445 |
Boc-a-Me-DL-Val-OH |
A-4145.0001 |
Bachem |
1.0g |
EUR 139 |
Description: Sum Formula: C11H21NO4; CAS# [139938-00-4] |
Boc-a-Me-DL-Val-OH |
A-4145.0005 |
Bachem |
5.0g |
EUR 477 |
Description: Sum Formula: C11H21NO4; CAS# [139938-00-4] |
Rabbit Anti-Influenza A HA (H1N1, 1-343aa) (A/Wyoming/3/03) protein IgG, aff pure |
H3N22-A |
Alpha Diagnostics |
100 ul |
EUR 445 |
Fmoc-a-amino-DL-Gly(Boc)-OH |
A-4320.0001 |
Bachem |
1.0g |
EUR 273 |
Description: Sum Formula: C22H24N2O6; CAS# [176039-39-7] |
Fmoc-a-amino-DL-Gly(Boc)-OH |
A-4320.0005 |
Bachem |
5.0g |
EUR 998 |
Description: Sum Formula: C22H24N2O6; CAS# [176039-39-7] |
Rabbit Anti-Influenza A nucleoprotein (H1N1-NP/1-320aa) protein (A/06/California/2009) IgG, aff pure |
H1NP21-A |
Alpha Diagnostics |
100 ul |
EUR 445 |
Rabbit Anti-Influenza A HA2 (H5N1) (A/Vietnam/1203/2004 366-531aa, >95%, his-tag) protein IgG |
HA2H012-A |
Alpha Diagnostics |
100 ul |
EUR 445 |
Rabbit Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG, aff pure |
H3N23-A |
Alpha Diagnostics |
100 ul |
EUR 445 |
Goat Anti-human Serum Amyloid A (SAA) antiserum |
SAA11-A |
Alpha Diagnostics |
100 ul |
EUR 482 |
Goat Anti-Hepatitis A Virus (Strain HM175) IgG |
HAV11-A |
Alpha Diagnostics |
100 ul |
EUR 457 |
Monocyte/Granulocyte Antibody |
abx415381-01mg |
Abbexa |
0.1 mg |
EUR 453 |
|
Rabbit Anti-Human Calcineurin A-subunit IgG aff pure |
CALNA12-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Rabbit Anti-Mouse a-1-Acid Glycoprotein IgG, aff pure |
A1AG18-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Rabbit Anti-Rat a-1-Acid Glycoprotein IgG, aff pure |
A1AG19-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Rabbit Anti-Mouse prepro Orexin A IgG # 1, aff pure |
PPOX11-A |
Alpha Diagnostics |
100 ul |
EUR 482 |
Rabbit Anti-Rat/Human Orexin-A IgG # 1, aff pure |
OXA11-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Goat Anti-Green Fluorescent Proteins (GFP, A. victoria) protein, IgG |
GFP11-A |
Alpha Diagnostics |
100 ul |
EUR 408 |
Rabbit Anti-Human Apolipoprotein A-I protein IgG, aff pure |
APOA12-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Rabbit Anti-Mouse Apolipoprotein A-I protein IgG, aff pure |
APOA13-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Goat Anti-Human Apolipoprotein A-Il protein IgG, aff pure |
APOA21-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Rabbit Phosphorylase b kinase regulatory subunit alpha, liver is |
ELI-08786Ra |
Lifescience Market |
96 Tests |
EUR 928 |
Rabbit Anti-Influenza A hemeagglutinin A2 (HA2/stem or stalk domain/347-523aa) protein (A/Viet Nam/1203/2004(H5N1) IgG, aff pure |
HA28-A |
Alpha Diagnostics |
100 ul |
EUR 445 |
Rabbit Anti-Hemagglutinin HA1 Influenza A Virus (H11N2; A/duck/Yangzhou/906/2002) IgG |
H11N2-01-A |
Alpha Diagnostics |
100 ul |
EUR 482 |
Rabbit Anti-bovine Calcineurin (A+B subunits) protein, IgG, aff pure |
CALN11-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Rabbit Anti-Borrelia burgdorferi Osp-A protein (OspA) IgG, aff pure |
OSPA12-A |
Alpha Diagnostics |
100 ul |
EUR 482 |
Rabbit Anti-Hemagglutinin Influenza A Virus H1N1 H1 (H1N1) (A/New Caledonia/20/99) IgG |
H1N1-01-A |
Alpha Diagnostics |
100 ul |
EUR 482 |
Rabbit Anti-Rat neurite outgrowth inhibitor (Nogo-A) IgG #1, aff. pure |
NogoA11-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Goat Anti-Human/mouse/rat Insulin chain A peptide IgG, aff pure |
INSA21-A |
Alpha Diagnostics |
100 ul |
EUR 482 |
Monocyte/Granulocyte Antibody (FITC) |
abx415380-01mg |
Abbexa |
0.1 mg |
EUR 648 |
|
Monocyte/Granulocyte Antibody (RPE) |
abx415382-01mg |
Abbexa |
0.1 mg |
EUR 718 |
|
Rabbit Anti-Rat Epithelial Na Channel alpha (ENAC-a) IgG #1, Aff. Pure |
ENACa11-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Goat Anti-Human recombinant Fetuin A (Alpha-2 HS-Glycoprotein, AHSG, A2HS) IgG |
FETB12-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Rabbit Anti-Human Neurite outgrowth inhibitor (Nogo A-C) IgG #2, aff. pure |
NogoA12-A |
Alpha Diagnostics |
100 ug |
EUR 482 |
Rabbit Anti-Influenza A virus HA IgG (aff pure) |
AB-23091-A |
Alpha Diagnostics |
100ug |
EUR 482 |
Granulocyte Colony Stimulating Factor Antibody |
20-abx137008 |
Abbexa |
|
|
|
Granulocyte Marker(BM-1) Antibody |
BNCA0061-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against Granulocyte Marker(BM-1), APC conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNCA0062-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against Granulocyte Marker(BM-2), APC conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNCAP0061-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNCAP0061-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNCAP0062-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNCAP0062-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC810061-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF680R conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC810061-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF680R conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC810062-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF680R conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC810062-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF680R conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC880061-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF488A conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC880061-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF488A conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC880062-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF488A conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC880062-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF488A conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNCB0061-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-1), Biotin conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNCB0061-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-1), Biotin conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNCB0062-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-2), Biotin conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNCB0062-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-2), Biotin conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC550061-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF555 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC550061-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF555 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC550062-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF555 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC550062-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF555 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC470061-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF647 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC470061-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF647 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC470062-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF647 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC470062-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF647 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC940061-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF594 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC940061-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF594 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC940062-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF594 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC940062-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF594 conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNCP0061-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against Granulocyte Marker(BM-1), PerCP conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNCP0062-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against Granulocyte Marker(BM-2), PerCP conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNCR0061-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against Granulocyte Marker(BM-1), RPE conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNCR0062-250 |
Biotium |
250uL |
EUR 383 |
Description: Primary antibody against Granulocyte Marker(BM-2), RPE conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC040061-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF405S conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-1) Antibody |
BNC040061-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-1), CF405S conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC040062-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF405S conjugate, Concentration: 0.1mg/mL |
Granulocyte Marker(BM-2) Antibody |
BNC040062-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Granulocyte Marker(BM-2), CF405S conjugate, Concentration: 0.1mg/mL |
Protease is obtained by expression from the pDZ2087 plasmid in BL21 (DE3) cells. Proteolysis resulting from this product, cleaves a maltose-binding protein fusion to completion at a fusion-to-protease molar ratio of 50:1.