A simplified method for producing laboratory grade recombinant TEV protease from E. coli.

The tobacco etch virus (TEV) protease has become a popular choice for cleaving fusion proteins because of its high stringency in sequence recognition. Procedures for isolating recombinant protein from the cytoplasm of E. coli require rupturing of the cell wall via enzymatic treatment combined with sonication or French press.

Here we present an expedited method for producing laboratory-grade TEV protease in E. coli using a freeze-thaw method, followed by purification with immobilized metal affinity chromatography.

Porcine IS ELISA Kit
EPI0046	Abclonal	96Tests	EUR 521

Rat IS ELISA Kit
ERI0046	Abclonal	96Tests	EUR 521

Rabbit IS ELISA Kit
ERTI0046	Abclonal	96Tests	EUR 521

Anserini IS ELISA Kit
EAI0046	Abclonal	96Tests	EUR 521

Goat IS ELISA Kit
EGTI0046	Abclonal	96Tests	EUR 521

Canine IS ELISA Kit
ECI0046	Abclonal	96Tests	EUR 521

Chicken IS ELISA Kit
ECKI0046	Abclonal	96Tests	EUR 521

Human IS ELISA Kit
EHI0046	Abclonal	96Tests	EUR 521

Bovine IS ELISA Kit
EBI0046	Abclonal	96Tests	EUR 521

IS ELISA KIT|Human
EF007501	Lifescience Market	96 Tests	EUR 689

Guinea Pig IS ELISA Kit
EGI0046	Abclonal	96Tests	EUR 521

Rabbit Anti-Mouse Granulocyte Colony Stimulating Factor (GCSF, G-CSF) protein IgG, aff pure
GCSF11-A	Alpha Diagnostics	100 ug	EUR 482

Rabbit Anti-Human Granulocyte Colony Stimulating Factor (GCSF, G-CSF) protein IgG, aff pure
GCSF12-A	Alpha Diagnostics	100 ug	EUR 482

Goat Anti-Human Apolipoprotein A-IV A(ApoA4) peptid IgG, aff pure
APOA45-A	Alpha Diagnostics	100 ul	EUR 482

Human Serum Indoxyl Sulfate (IS) ELISA Kit
abx257961-96tests	Abbexa	96 tests	EUR 746

Human IS(serum indoxyl sulfate) ELISA Kit
EH4913	FN Test	96T	EUR 567.6
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75 ng/ml

Rabbit Anti-human MART-1/Melanocyte Differentiation-A/Melan-A peptide (CT) IgG
MART12-A	Alpha Diagnostics	100 ul	EUR 445

Boc-a-Me-DL-Val-OH
A-4145.0001	Bachem	1.0g	EUR 139
Description: Sum Formula: C11H21NO4; CAS# [139938-00-4]

Boc-a-Me-DL-Val-OH
A-4145.0005	Bachem	5.0g	EUR 477
Description: Sum Formula: C11H21NO4; CAS# [139938-00-4]

Rabbit Anti-Influenza A HA (H1N1, 1-343aa) (A/Wyoming/3/03) protein IgG, aff pure
H3N22-A	Alpha Diagnostics	100 ul	EUR 445

Fmoc-a-amino-DL-Gly(Boc)-OH
A-4320.0001	Bachem	1.0g	EUR 273
Description: Sum Formula: C22H24N2O6; CAS# [176039-39-7]

Fmoc-a-amino-DL-Gly(Boc)-OH
A-4320.0005	Bachem	5.0g	EUR 998
Description: Sum Formula: C22H24N2O6; CAS# [176039-39-7]

Chicken Anti-Protein A IgG, aff pure
PRTA11-A	Alpha Diagnostics	1 mg	EUR 482

Goat Anti-Protein-A IgG aff pure
PRTA12-A	Alpha Diagnostics	0.1 ml	EUR 408

Chicken Anti-Protein-A IgG aff pure
PRTA13-A	Alpha Diagnostics	100 ug	EUR 408

Rabbit Anti-Influenza A nucleoprotein (H1N1-NP/1-320aa) protein (A/06/California/2009) IgG, aff pure
H1NP21-A	Alpha Diagnostics	100 ul	EUR 445

Rabbit Anti-Influenza A HA2 (H5N1) (A/Vietnam/1203/2004 366-531aa, >95%, his-tag) protein IgG
HA2H012-A	Alpha Diagnostics	100 ul	EUR 445

Rabbit Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG, aff pure
H3N23-A	Alpha Diagnostics	100 ul	EUR 445

Goat Anti-human Serum Amyloid A (SAA) antiserum
SAA11-A	Alpha Diagnostics	100 ul	EUR 482

Rabbit Anti-Streptococcus A IgG, aff pure, #2
STA12-A	Alpha Diagnostics	1 mg	EUR 286

Goat Anti-Hepatitis A Virus (Strain HM175) IgG
HAV11-A	Alpha Diagnostics	100 ul	EUR 457

Monocyte/Granulocyte Antibody
abx415381-01mg	Abbexa	0.1 mg	EUR 453

Rabbit Anti-Human Calcineurin A-subunit IgG aff pure
CALNA12-A	Alpha Diagnostics	100 ug	EUR 482

Chicken Anti-Human+Mouse IgG+A+M IgG/Y
HGAM11-A	Alpha Diagnostics	100 ul	EUR 482

Hematoxylin, Weigert's Iron (Part A)
HWI-A-250	ScyTek Laboratories	250 ml	EUR 116

Rabbit Anti-Mouse a-1-Acid Glycoprotein IgG, aff pure
A1AG18-A	Alpha Diagnostics	100 ug	EUR 482

Rabbit Anti-Rat a-1-Acid Glycoprotein IgG, aff pure
A1AG19-A	Alpha Diagnostics	100 ug	EUR 482

Rabbit Anti-Mouse prepro Orexin A IgG # 1, aff pure
PPOX11-A	Alpha Diagnostics	100 ul	EUR 482

Rabbit Anti-Rat/Human Orexin-A IgG # 1, aff pure
OXA11-A	Alpha Diagnostics	100 ug	EUR 482

Goat Anti-Green Fluorescent Proteins (GFP, A. victoria) protein, IgG
GFP11-A	Alpha Diagnostics	100 ul	EUR 408

Rabbit Anti-Human Apolipoprotein A-I protein IgG, aff pure
APOA12-A	Alpha Diagnostics	100 ug	EUR 482

Rabbit Anti-Mouse Apolipoprotein A-I protein IgG, aff pure
APOA13-A	Alpha Diagnostics	100 ug	EUR 482

Goat Anti-Human Apolipoprotein A-Il protein IgG, aff pure
APOA21-A	Alpha Diagnostics	100 ug	EUR 482

Rabbit Phosphorylase b kinase regulatory subunit alpha, liver is
ELI-08786Ra	Lifescience Market	96 Tests	EUR 928

Rabbit Anti-Influenza A hemeagglutinin A2 (HA2/stem or stalk domain/347-523aa) protein (A/Viet Nam/1203/2004(H5N1) IgG, aff pure
HA28-A	Alpha Diagnostics	100 ul	EUR 445

Rabbit Anti-Hemagglutinin HA1 Influenza A Virus (H11N2; A/duck/Yangzhou/906/2002) IgG
H11N2-01-A	Alpha Diagnostics	100 ul	EUR 482

Rabbit Anti-bovine Calcineurin (A+B subunits) protein, IgG, aff pure
CALN11-A	Alpha Diagnostics	100 ug	EUR 482

Rabbit Anti-Borrelia burgdorferi Osp-A protein (OspA) IgG, aff pure
OSPA12-A	Alpha Diagnostics	100 ul	EUR 482

Rabbit Anti-Hemagglutinin Influenza A Virus H1N1 H1 (H1N1) (A/New Caledonia/20/99) IgG
H1N1-01-A	Alpha Diagnostics	100 ul	EUR 482

Rabbit Anti-Rat neurite outgrowth inhibitor (Nogo-A) IgG #1, aff. pure
NogoA11-A	Alpha Diagnostics	100 ug	EUR 482

Goat Anti-Human/mouse/rat Insulin chain A peptide IgG, aff pure
INSA21-A	Alpha Diagnostics	100 ul	EUR 482

Monocyte/Granulocyte Antibody (FITC)
abx415380-01mg	Abbexa	0.1 mg	EUR 648

Monocyte/Granulocyte Antibody (RPE)
abx415382-01mg	Abbexa	0.1 mg	EUR 718

Rabbit Anti-Rat Epithelial Na Channel alpha (ENAC-a) IgG #1, Aff. Pure
ENACa11-A	Alpha Diagnostics	100 ug	EUR 482

Goat Anti-Human recombinant Fetuin A (Alpha-2 HS-Glycoprotein, AHSG, A2HS) IgG
FETB12-A	Alpha Diagnostics	100 ug	EUR 482

Rabbit Anti-Human Neurite outgrowth inhibitor (Nogo A-C) IgG #2, aff. pure
NogoA12-A	Alpha Diagnostics	100 ug	EUR 482

Rabbit Anti-Influenza A virus HA IgG (aff pure)
AB-23091-A	Alpha Diagnostics	100ug	EUR 482

Granulocyte Colony Stimulating Factor Antibody
20-abx137008	Abbexa	EUR 356.00 EUR 523.00	0.5 mg 1 mg

Granulocyte Marker(BM-1) Antibody
BNCA0061-250	Biotium	250uL	EUR 383
Description: Primary antibody against Granulocyte Marker(BM-1), APC conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNCA0062-250	Biotium	250uL	EUR 383
Description: Primary antibody against Granulocyte Marker(BM-2), APC conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNCAP0061-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNCAP0061-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNCAP0062-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNCAP0062-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC810061-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF680R conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC810061-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF680R conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC810062-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF680R conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC810062-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF680R conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC880061-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF488A conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC880061-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF488A conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC880062-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF488A conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC880062-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF488A conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNCB0061-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), Biotin conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNCB0061-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), Biotin conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNCB0062-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), Biotin conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNCB0062-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), Biotin conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC550061-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF555 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC550061-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF555 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC550062-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF555 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC550062-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF555 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC470061-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF647 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC470061-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF647 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC470062-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF647 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC470062-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF647 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC940061-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF594 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC940061-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF594 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC940062-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF594 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC940062-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF594 conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNCP0061-250	Biotium	250uL	EUR 383
Description: Primary antibody against Granulocyte Marker(BM-1), PerCP conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNCP0062-250	Biotium	250uL	EUR 383
Description: Primary antibody against Granulocyte Marker(BM-2), PerCP conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNCR0061-250	Biotium	250uL	EUR 383
Description: Primary antibody against Granulocyte Marker(BM-1), RPE conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNCR0062-250	Biotium	250uL	EUR 383
Description: Primary antibody against Granulocyte Marker(BM-2), RPE conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC040061-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-1), CF405S conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-1) Antibody
BNC040061-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-1), CF405S conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC040062-100	Biotium	100uL	EUR 199
Description: Primary antibody against Granulocyte Marker(BM-2), CF405S conjugate, Concentration: 0.1mg/mL

Granulocyte Marker(BM-2) Antibody
BNC040062-500	Biotium	500uL	EUR 544
Description: Primary antibody against Granulocyte Marker(BM-2), CF405S conjugate, Concentration: 0.1mg/mL

Protease is obtained by expression from the pDZ2087 plasmid in BL21 (DE3) cells. Proteolysis resulting from this product, cleaves a maltose-binding protein fusion to completion at a fusion-to-protease molar ratio of 50:1.